GNG5 and Alzheimer disease: To explore the effect of GNG5 in vivo, we designed to establish neuronal targeted GNG5 overexpressing and GNG5 knockdown AD model mice by engineered EV deliver of exogenous GNG5 or siGNG5 [18], and to investigate Aβ pathology in brains and the cognition-related behavioral changes of mice (Fig. 3A).