To further explain the variation of GNG5 in EVs of AD patients, we enriched neuron-derived extracellular vesicles (NDEVs) from 5 cognitively normal participants (CN, aged, 86.1 ± 6.3 yr), and 5 patients with AD (aged, 88.4 ± 7.4 yr). The gene discussed is GNG5; the disease is Alzheimer disease.