To ascertain the interaction between GNG5 and PS1 in pathophysiological conditions, we stained paraffin sections of eight brain regions from two postmortem brains diagnosed as AD with “H” pathological level (A3B3C3) (Figs. 5D and S7A), and hippocampal–entorhinal paraffin sections from six NC and two pathological AD donors (Fig. S7B). This evidence concerns the gene PSEN1 and Alzheimer disease.