Here, we established HeLa stable cells expressing four TRIM32 mutants, including C39/44A that inactivates E3 ubiquitin ligase activity, P130S, which is associated with Bardet-Biedl syndrome (BBS11), and two well-characterized mutants R394H and D487N that are associated with Limb-girdle muscular dystrophy (Fig 7A). Here, TRIM32 is linked to Bardet-Biedl syndrome.