However, the late treatment of these diabetic rats (24 weeks into diabetes) with alagebrium for 8 weeks reduced the AGE (CML) levels to baseline but did not mitigate glomerular fibrosis by a statistically significant amount, nor were TGF-β and collagen IV within the renal cortex reduced by statistically significant amounts (Forbes et al., 2003). This evidence concerns the gene TGFB1 and diabetes mellitus.