The cytokine analysis of supernatant from the CaSki killing assay showed significantly higher levels of interleukin (IL)-2, interferon (IFN)-γ, granzymes A and B, perforin, and granulysin, which were released from CAR-T cells when cocultured with tumor cells for 24 or 48 h at 12:1 E/T ratios, while minimal cytokine production was observed from mock T cells (Figure 4F). This evidence concerns the gene PRF1 and neoplasm.