Next, SAMD5 mRNA expression was determined in non-cancerous breast epithelial cells (MCF-10A and MCF-12A) and breast carcinoma cell lines (MDA-MB-231, HCC1937, and BT-483); Figure 5D shows that compared with MCF-10A and MCF-12A, SAMD5 mRNA expression was significantly downregulated in MDA-MB-231, HCC1937, and BT-483 cell lines. The gene discussed is SAMD5; the disease is breast carcinoma.