However, post‐radiotherapy treatment of the organoids with L‐NAME led to a decrease in both the proportion of CD133‐positive tumour cells (Figure 6H) and Notch1 protein levels (Figure 6I), indicating that NO regulation could control the CSC pool by targeting Notch1 protein in NSCLC PDOs. The gene discussed is PROM1; the disease is non-small cell lung carcinoma.