Although the presence of microvilli and the expression of apically located proteins was not investigated, expression of intestinal stem cell marker LGR5, goblet cell marker MUC2, and enteroendocrine cell marker CHGA could not be detected by qPCR and ICC, respectively, (data not shown), suggesting that the differentiated IPEC-J2 spheroids exclusively consisted of enterocytes. Here, MUC2 is linked to intrahepatic cholangiocarcinoma.