In order to investigate if other cell types than enterocytes are present in the differentiated IPEC-J2 spheroids, the expression of intestinal stem cell marker G-protein-coupled receptor 5 (LGR5), goblet cell marker mucin 2 (MUC2), and enteroendocrine cell marker chromogranin A (CHGA) was measured using qPCR and ICC, respectively. The gene discussed is LGR5; the disease is intrahepatic cholangiocarcinoma.