RHOB and bladder transitional cell carcinoma: Additionally, immunofluorescence staining was used to detect the localization of integrin β1, and the results showed that the abundance of integrin β1 on the cell membrane decreased after RhoB overexpression or MVA pathway inhibition by simvastatin and ZOL in BLCA cells (Fig. 7e, f and Supplementary Fig. 12d, e), consistent with our western blot findings.