SLC2A1 and glioblastoma: Subsequently, we conducted validation at both the nucleic acid and protein levels, confirming a reduction in GLUT1 and GLUT6 expression (Fig. 3a–d).Transfection of U87 and T98G cells with sh-GLUT1 and sh-GLUT6 plasmids was performed, and one-step TRUNAL experiments revealed that the decrease in GLUT1 or GLUT6 expression led to an increase in apoptosis in glioblastoma cells (Fig. 3e, f).