TNF and gastric cancer: To evaluate the inflammatory responses induced by compound LES-6400 (1 μM), DOX (1 μM), trastuzumab (10 μg/mL), and their combinations, we treated AGS gastric cancer cells with these compounds for 24 h and then evaluated the secretion of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12p70, and TNF) by flow cytometry (Figure 10).