In summary, our study, for the first time, confirms a causal relationship between choline metabolites and cholecystitis, mediated through the protective action of LDL, and identifies the possible loci responsible for this causal relationship, suggesting a role for ABCG8 in the development of non-calculous cholecystitis, which provides valuable information for probing its molecular mechanisms. The gene discussed is ABCG8; the disease is Cholecystitis.