Applied to insomnia, the approach identified 25 interactions between 32 human proteins absent in known knowledge bases, including regulatory interactions between MAOA and 5-HT2C, binding between ADAM22 and 14-3-3 proteins, which is implicated in neurological diseases, and a circadian regulatory loop involving RORB and NR1D1. This evidence concerns the gene NR1D1 and insomnia.