In another in vivo study by Li, G., et al. in male Wistar rats in which diethylnitrosamine (200 mg/kg) was used to induce liver cancer, treatment with 100 μM/kg of prunetin for 16 weeks delayed liver cancer growth, downregulated cyclin-D1 protein expression, and upregulated Bcl-2, Bax, caspase-3, and caspase-9 gene expression [79]. This evidence concerns the gene CASP3 and liver cancer.