To verify whether MDH2 affects HCC cell ferroptosis via GPX4, we overexpressed GPX4 in MDH2-knockdown HCCLM3 and HLE cells, and our cell death analysis revealed that GPX4 overexpression significantly rescued MDH2-knockdown HCC cell death induced by RSL3 (Figure 6C,D). Here, MDH2 is linked to hepatocellular carcinoma.