Figure 2A is the schematic diagram of the study design. The skins of the mice were sensitized with DNCB every 5 days for five times starting from day 0. Mice in the DNCB + BSEC group were treated with BSEC daily from day 0 to day 25. ELISA results showed that DNCB-mediated skin sensitization dramatically increased IL-6 levels in the skin tissues compared to the negative control group (Figure 2B); however, the increase in IL-6 was significantly suppressed by BSEC treatment. TNF-α results showed a similar trend as well (Figure 2C). The gene discussed is IL6; the disease is skin sensitization.