NRXN1 and colorectal carcinoma: To validate the transcriptome analysis findings, we investigated the effect of NRXN1 knockdown on the EMT signaling pathway in CRC cells, and observed increased ZEB1, N-cadherin, snail, slug, and twist expression in siNRXN1-transfected CRC cells, whereas E-cadherin levels decreased at the mRNA and protein levels (Figure 5A,B).