This suggests the utility of customised 22-protein dot blotting kits for the detection of BM proteins (SPARC, HSPG2, FN1, TIMP1, LAMB1, LAMA4, LAMC1, LOXL2, NID1, FBN1, AGRN, COL4A1, COL4A2), innate immune response molecules (e.g., soluble CD59, IL-6, IL-8, MCP-1/CCL2, MIF, CXCL1, and MIP-3α/CCL20), and pro- or anti-thrombotic molecules (serpin E1/PAI-1 and uPAR) in cell culture supernatant for the detection of endothelial dysfunction in vitro. Here, CXCL1 is linked to endothelial dysfunction.