CD40LG and infectious disease: The infectious disease testing included three dogs with a negative Neospora caninum serology IFA, three dogs with a negative Toxoplasma gondii antibody titer (IgG + IgM), two dogs with negative Hepatozoon PCR, and one dog for each of the following: negative canine distemper virus antibody titer (IgG + IgM), negative Ehrlichia canis antibody titer (IFA), and negative Rocky Mountain spotted fever antibody titer (IFA).