Additionally, TBX5 has been verified to drive the expression of multiple genes, including SCN5A, PITX2, GJA1, and RyR2 [98,99,100], and mutations in SCN5A, PITX2, GJA1, and RyR2 have been reported to cause AF [2,49]. The gene discussed is PITX2; the disease is atrial fibrillation.