Sanger sequencing examination in all the pedigree members as indicated in Family AF-01 revealed that only the nonsense variation of chr6: 21,594,976C>T (GRCh37.p13/hg19: NC_000006.11), equivalent to chr6: 21,594,745C>T (GRCh38.p14/hg38: NC_000006.12) or NM_003107.3: c.211C>T; p.(Gln71*) in SOX4, was verified to be in co-segregation with AF in the whole pedigree. The gene discussed is SOX4; the disease is atrial fibrillation.