Strikingly, at interphase, hepatic (LX-2 and HepG2) and colon cancer (HCT116-p53+/+ and HCT116-p53−/−) (human) cell lines were characterized by a mainly cytoplasmic pattern (absence of nuclear staining) of PACT distribution, while, at mitosis, PACT maintained its strong, albeit diffuse, immunodetection signal that was being dispersed throughout all the mitotic cells examined (Figure 8). The gene discussed is PRKRA; the disease is colonic neoplasm.