NSDHL and neoplasm: After injection with 1 × 106 cells of shCtrl and shNSDHL MCF-7 spheroids, NSDHL knockdown resulted in a significant reduction in tumor volume (shCtrl vs. shNSDHL; n = 6, 527.79 ± 270.95 vs. 232.09 ± 113.86 mm3 at 49 days, P = 0.033 and 733.06 ± 405.53 vs. 270.04 ± 152.20 mm3 at 56 days, P = 0.026, respectively) and tumor weights (shCtrl vs. shNSDHL; n = 6, 0.34 ± 0.21 g vs. 0.13 ± 0.08 g at 56 days, P = 0.043) (Fig. 4J-L).