Western blotting revealed that RU486 treatment significantly decreased GR activation while increasing nuclear translocation of MR in the PVN of the hypothalamus of MPS-treated mice (Fig. 2h–j), suggesting that GR inhibition in the PVN of the MPS-treated mice was sufficient to restore the balance of nuclear GR and MR (Fig. 2k). Here, NR3C2 is linked to mucopolysaccharidosis.