To unravel the intricate mechanisms governing the gene expression program influenced by EZH2-mediated histone modifications in HCC, we performed CUT&Tag experiments to map H3K27me3 in the HepG2 cell line under conditions of EZH2 inhibition using tazemetostat, as well as EZH2 knockdown. Here, EZH2 is linked to hepatocellular carcinoma.