Since the driver mutations in the tumour of subject#168 included PIK3CA c.3140A > G (VAF: 0.169), PTEN c.389G > A (VAF: 0.166) and PTEN c.981del (VAF: 0.125), the second hit of the PTEN deletion might have transformed the non-neoplastic clone to the premalignant and/or malignant clones. This evidence concerns the gene PIK3CA and neoplasm.