Immunoblotting showed that, compared with Ad-GFP infection, Ad-Psmb8 overexpression substantially decreased the total Drp1 and phosphorylated (p)-Drp1 (S616) protein levels and increased the Mfn1 and Mfn2 protein levels following H/R stimulation (Fig. 5E). This evidence concerns the gene MFN2 and infection.