Unexpectedly, when the downstream response to released IL-33 was measured 24 hr later (Figure 2C), IL-33 tethering did not correlate with suppression of IL-33-induced responses: while HpARI2 suppressed and HpARI3 amplified eosinophilia, ILC2 activation (as measured by surface CD25 or cell size by median FSC), and IL-5 release (Figure 2D–H), HpARI2:3 could not suppress IL-33 responses despite tethering of IL-33 at the 15 min timepoint (Figure 2B). Here, IL5 is linked to Increased total eosinophil count.