TNF and melanoma: We treated various cell types, including Huh-7 (hepatoma), J774A.1 (macrophage), B16F10 (melanoma), and primary mouse splenocytes, with different cell death inducers such as staurosporine (apoptosis), rapamycin (autophagy), erastin (ferroptosis), TNF-α (necroptosis), and nigericin (pyroptosis).