Stimulation with PMA and ionomycin had a greatly reduced effect on the percentage of TIM-3 positivity in glioblastoma patients’ total T cells (reduced to 63% of unstimulated level), CD4+ T cells (reduced to 80% of unstimulated level), CD8+ T cells (reduced to 46% of unstimulated level), Tregs (increased to 109% of unstimulated level), and NK cells (reduced to 81% of unstimulated level) (Figure 2A–E and Figure S3A–E). This evidence concerns the gene CD4 and glioblastoma.