In order to confirm our in vitro post GC B-cell model of EBV-associated cHL, where the LMP1 oncogene mediates multinuclearity through down-regulation of TRF2, we further developed and adapted our 3D combined quantitative TRF2-telomere immuno Q-FISH protocol (3D TRF2/Telo-Q-FISH) [29,45,47,67] to monolayers of H- and RS-cells including the surrounding lymphocytes [69]. This evidence concerns the gene TERF2 and classic Hodgkin lymphoma.