To further elucidate such immune evasive capacity and specifically the role of EVs and molecules vehiculated by such soluble mediators, we i) analyzed vesicles isolated from BM of NB patients in comparison with healthy subjects; ii) assessed HLA-G, PD-1 and PD-L1 expression on such EVs; iii) evaluated the contribution of HLA-G and PD-1/PD-L1 axis on immune-regulatory properties of such EVs. The gene discussed is HLA-G; the disease is neuroblastoma.