To get a better insight into the differential abundance of circulating CD27, we measured CD27 expression in different peripheral blood compartments using an independent EV characterizationcohort of another n = 45 HCC patients (all stages): whole (unprocessed) serum (median CD27 concentration: 2319.05 pg/ml), large EV fractions (i.e., microparticles) (median: 290.6 pg/ml), small EV fraction (median: 657.57 pg/ml) and EV-depleted serum (median: 1805.76 pg/ml). This evidence concerns the gene CD27 and hepatocellular carcinoma.