After reverse transcription, the expression of AD- and ferroptosis-related genes [amyloid precursor protein (App), ferritin (Fth1), fatty acid CoA ligase 4 (Acsl4), glutathione peroxidase 4 (Gpx4), arachidonate 15-lipoxygenase (Alox15)] was quantified by RT-PCR. The gene discussed is APP; the disease is Alzheimer disease.