The functional and pathway analyses were corroborated by the Qiagen IPA tool (Fig. 2f, Fig. S7, Supplementary file 4), although it indicated that downregulation of EIF2 signaling (topmost) plays a major role in MDS as well as multiple dysfunctional signaling pathways (i.e., integrin, actin cytoskeletal signaling, Rho family GTPase signaling, and RHOGDI signaling). The gene discussed is ARHGDIA; the disease is myelodysplastic syndrome.