Western blot analysis revealed that levels of apoptotic proteins (Cleaved-caspase 3 and Bax), Cleaved PARP/PARP, and Cleaved caspase 3/caspase 3 were elevated, while anti-apoptotic protein levels (Bcl-2) were decreased in HCC cells (Figure 3C–3E). The gene discussed is CASP3; the disease is hepatocellular carcinoma.