Of them, HFD-induced obese mouse model is closer to human MASLD status, characterized by obesity (body weight gain), insulin resistance, intracellular TG accumulation in the liver, hepatocellular fat vacuoles, and elevations in serum levels of ALT, AST, TC, and LDL-c as shown in this study. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.