Further evidence that suggested the presence of hepatic steatosis included notable intracellular lipid accumulation and vast fat vacuoles and lipid droplets in the hepatocytes as measured with oil red O staining and presence of more TG-deposited hepatocytes illustrated by HE staining (Figures 1E, F), in parallel with significant elevations in liver TG content as well as serum levels of ALT, AST, TC, HDL-c, and LDL-c (Figures 1G–J). Here, GPT is linked to fatty liver disease.