NOS3 and endothelial dysfunction: Consistent with Jan-On et al. [19], this experiment found that chronic treatment with L-NAME (50 mg/kg BW/day) for 7 weeks exhibited a blockade of NO production, induced hypertension, and endothelial dysfunction, as shown by an attenuated vascular response to the endothelium-dependent vasodilator ACh and reduced plasma nitrate/nitrite levels and decreased aortic eNOS protein expression.