Immunohistochemically, ALM typically shows negative staining for epithelial membrane antigen (EMA), serum acidic binding protein S100, glial fibrillary acidic protein (GFAP), and desmin, while smooth muscle actin (SMA) and either CD34 or CD31 are markers for muscle cells and vascular endothelial cells, respectively. This evidence concerns the gene DES and acral lentiginous melanoma.