In the present study, we investigated the potential prognostic contribution of SMG-1 to HNSCC in multiple aspects, includingin vitro andin vivo experiments (Supplementary Methods), to examine the impact ofSMG-1 silencing on HNSCC cell growth and the response to radiotherapy and retrospectively analyzed the survival of SMG-1-positive and SMG-1-negative HNSCC patients with and without radiotherapy. The gene discussed is SMG1; the disease is head and neck squamous cell carcinoma.