MYC and colorectal cancer: Finally, pemigatinib did not result in MYC ecDNA loss in the COLO 320DM colorectal cancer cell line, which does not contain FGFR2 ecDNAs (Fig. 1c and Extended Data Fig. 10d), showing that the loss of MYC ecDNAs in SNU16m1 cells is specifically due to the coupling with FGFR2 ecDNAs.