We firstly explored the genetic alteration of P4HA3 in TCGA pan-cancer atlas dataset by using the cBioPortal online database, we found that the overall alteration frequency of P4HA3 was widely high in various of cancers, P4HA3 had the highest genetic alteration frequency in SKCM, ESCA, OV, UCEC, HNSCC and BRCA (Fig 2A). This evidence concerns the gene P4HA3 and cancer.