PDCD1 and neoplasm: More importantly, the tumor tissues from needle biopsy of two TNBC patients before immunotherapy were conducted for WB and IHC staining to detect the expression of P4HA3, Ki-67 and PD-1, we found that the expression of P4HA3, Ki-67 and PD-1 in second TNBC patient was significantly higher than the first TNBC patient (Fig 7H and 7I).