In the UMAP generated from all Lin-CD34+CD38-/low primitive cells, we identified three coarse clusters, where two consisted of primitive CML cells with representation from all patients (cluster 2–3), and one represented Lin-CD34+CD38-/low primitive cells from nBM which were included as negative control (cluster 1 Figure 4—figure supplement 1B–C). This evidence concerns the gene CD38 and chronic myelogenous leukemia, BCR-ABL1 positive.