By applying the BCR::ABL1 signatures also to the Lin-CD34+ primitive cells (Figure 4—figure supplement 4), we could visualize the relative expression within the Lin-CD34+ UMAPs in relation to the cells BCR::ABL1 status and further confirm that the primitive fraction, previously captured by the established CML stem cell markers CD25, CD26, and CD93, consisted of specifically BCR::ABL1+ LSCs (Figure 5B–C, Figure 5—figure supplement 1 and Figure 5—figure supplement 2A). This evidence concerns the gene CD34 and chronic myelogenous leukemia, BCR-ABL1 positive.