Retinoschisis is predominantly observed in the INL of clinical XLRS patients[24] and RS1 is expressed in bipolar cells and photoreceptors in the retina.[25] Thus, we performed immunofluorescence staining to verify the expression and localization of the RS1 protein and the integrity of bipolar cells in wild‐type and Rs1emR209C retinas prior to scRNA‐seq. The gene discussed is RS1; the disease is retinoschisis.