GZMB and obesity due to melanocortin 4 receptor deficiency: Through GSEA analysis, we found that in obesity, GZMB is primarily involved in antigen processing and presentation, natural killer cell-mediated cytotoxicity, cell adhesion molecules (CAMs), leukocyte transendothelial migration, cytokine-cytokine receptor interaction, chemokine signaling pathway, JAK-STAT signaling pathway, and FCεRI signaling pathway (Supplementary Figure S2B).