The heatmap reveals that GZMB upregulates the expression levels of monocytes, dendritic cells, and eosinophils in obesity, MSR1 upregulates the expression levels of macrophages and natural killer cells in obesity, and IL6R upregulates the expression levels of dendritic cells in obesity (Supplementary Figure S2A). Here, GZMB is linked to obesity due to melanocortin 4 receptor deficiency.