To explore the physiologic function of USP3- SMARCA5 axis in the DNA damage process, we detected γ-H2AX foci formation in USP3 or SMARCA5 silenced cells after exposure to 2.5 nM docetaxel (Supplementary Fig. S3A, B showed 2.5 nM docetaxel could induce obvious morphological changes of two PCa cells). The gene discussed is H2AX; the disease is posterior cortical atrophy.