EBV antibody and EBV-DNA are commonly used clinical NPC screening items, which positivity rate and DNA copy number have definite correlations with the TNM stage and prognosis of patients with NPC, but the specificity and sensitivity need to be improved.14, 15, 16 In this study, the expression levels of HOTTIP in the serum of NPC patients were analyzed to investigate the role of HOTTIP in the clinicopathological features and prognosis evaluation of NPC patients. This evidence concerns the gene HOTTIP and nasopharyngeal carcinoma.