To evaluate the impact of SLITRK4 overexpression on global changes in immune cells from the TME, we performed single‐cell RNA sequencing (scRNA‐seq) in CT26 CRC liver tumors by injecting with CT26, CT‐26‐shRNA, and CT26‐ovSLITRK4 cells into the spleens of BALB/c mice. Here, SLITRK4 is linked to colorectal carcinoma.