(in section C21, L21, C17 and L17, for CD68 p value = 1.65 × 10–41, 8.70 × 10–45, 7.96 × 10–22, 2.10 × 10–34, for CD4 p-value = 6.44 × 10–28, 5.52 × 10–25, 1.26 × 10–8, 7.65 × 10–35, and for CTLA4 p-value = 2.82 × 10–19, 1.58 × 10–11, 6.65 × 10–16, 6.71 × 10–24, respectively) (Supplementary File), therefore, the clustered spots with those genes could be represented as THC in CRC and metastasis of the matched liver. The gene discussed is CTLA4; the disease is colorectal carcinoma.