Flow cytometry analysis revealed a notable increase in the CD8+ T cell population, macrophages (F4/80), dendritic cells (CD11c), B cells (B220), and NK cells (CD49b) in the tumor tissue microenvironment of the TTNP group compared to those in the PBS and blank NP groups (Figure 4a,b; Figure S4, Supporting Information). The gene discussed is ITGA2; the disease is neoplasm.