We established a mouse model of HCC cell lung metastasis by using tail vein injection of SK-HEP-1-Luc2-tdT cells with AAV-vector, AAV-p65/S536A, or AAV-p65/S536D plasmid solution to validate the effect of RelA/p65 Ser536 phosphorylation on tumor metastasis (Figure 2A). This evidence concerns the gene RELA and hepatocellular carcinoma.