This study indicated that GJB2, SLC26A4, MYO15A, OTOF, and CDH23 showed greater contribution in cases of autosomal recessive deafness (Svidnicki et al., 2015) However, this study screened only previously known variants and NGS, as performed in this study, allowed us to identify ten novel variants and solved additional cases. This evidence concerns the gene GJB2 and hearing loss, autosomal recessive.